g(sh)īI(xin)
˿̖(ho)R(sh)eww(SRP)ԇвE
1. (bio)(zhn)ƷϡcӘӣø(bio)O(sh)(bio)(zhn)Ʒ10diһڶзքeӘ(bio)(zhn)Ʒ100μlȻdiһڶмӘ(bio)(zhn)ƷϡҺ50μl;Ȼdiһڶиȡ100μlքeӵ͵ĿڵĿքeӘ(bio)(zhn)ƷϡҺ50μl;Ȼڵ͵Ŀȸȡ50μlٸȡ50μlքeȡ50μlքeӵڰ˿ڵߡڰ˿зքeӘ(bio)(zhn)ƷϡҺ50μlĵڰ˿мӵڵзքeӘ(bio)(zhn)ƷϡҺ50ul;ĵиքeȡ50μlӵھʮڵھŵʮքeӘ(bio)(zhn)ƷϡҺ50μlĵھŵʮиȡ50μl(ϡጺӘ50μlȷքe180 ng/L120 ng/L 60 ng/L30 ng/15 ng/L)
2. ӘӣքeO(sh)հ(հ(du)տײӘƷø(bio)ԇͬ)y(c)Ʒø(bio)ϴy(c)ƷȼӘƷϡҺ40μlȻټӴy(c)Ʒ10μl(ƷKϡጶȞ5)ӘӌƷø(bio)ײM|ױpp΄(dng)
3. ÷Ĥ3730
4. Һ30(48T20)sϴҺsˮ30(48T20)ϡጺá
5. ϴ죺СĽҵĤȥҺw˦ɣÿӝMϴҺo30ȥ؏(f)5ĸ
6. øÿø(bio)ԇ50μlհ׳
7. ͬ3
8. ϴ죺ͬ5
9. @ɫÿȼ@ɫA50μlټ@ɫB50μlppʎ37ܹ@ɫ15.
10. KֹÿӽKֹҺ50μlKֹ(yng)(˕r(sh){(ln)ɫD(zhun)Sɫ)
11. y(c)Կհ{(dio)㣬450nmL(zhng)y(c)(ODֵ) y(c)(yng)ڼӽKֹҺ15
˿̖(ho)R(sh)ewwԇбһֱҕ|(zh)ƞI(y)I(y)(jng)(zhng)IJ˾ڽ(jng)I(yng)ʼKУo(j)ط(yn)ɼԴڳГ(dn)I(y)(bio)(zhn)^Ӳ|(zh)̓(yu)ķ(w)So(h)չЈ(chng)^ȵĝM͑c͑ĹA҂İl(f)չĿ(bio)!εĺ҂?c)c\(chng)(chung)õδ
ԭ(chung)ߣƼ˾
P(gun)]a(chn)Ʒ
Ո(qng)ݔҪP(gun)I~
Ƽ˾ob^,ABI7500ð(lin),B(yng)ƿ,SBSaһ,0.1mlPCRι,0.1mlPCRB,300ulob^,4%M(x)̶Һ,ɹⶨPCRW(xu)ƽw,oȹ߅96PCR,Roche480PCR96װ,ɹⶨPCRùW(xu)Ĥ,Roche480o,6ELISAԇ,15mlxĹ,2.0mlixĹ,50ml(x)B(yng)ƿ,ob^,СIgG-HRP,ɭ200ul^,һҺ,1000ulVо^,һB(yng),96װ,ϷȾɫҺ,96ɲø(bio),2.0mlixĹ,ؼ(j)̥ţѪ,pRNAԇ,˼M(jn)0.2mlBPCR
ַ^(q)܇a(chn)I(y)@ Ԓ18502669006 棺 g(sh)֧֣xW(wng) L1411740